Background: Vitamin B status evaluation of patients with coeliac disease reveals that these patients are more prone to develop deficiencies of vitamin B1, B2, B6, folate and vitamin B12 as compared to healthy controls, all of which play an important role in homocysteine metabolism.
Objectives: To study the serum homocysteine levels in children with coeliac disease and to analyse in comparison to a few related markers.
Method: A cross-sectional study was done among 36 newly diagnosed children (44.4 % females) with coeliac disease (mean age: 7.72 ± 3.26 years); and 36 age-and-sex-matched controls. Serum concentrations of homocysteine, C-reactive protein (CRP), vitamin B12, and folic acid were determined after overnight fasting. Comparisons between the two groups were performed by Student’s t-test or Mann–Whitney U-test, for continuous variables. Pearson’s Chi-square test or Fisher’s exact test was used for categorical variables. Alpha level of 5% was considered significant.
Results: Biochemical assessment of the two groups revealed significantly increased serum levels of homocysteine (mean 14.1 ± 1.35 µmol/L) and CRP levels (mean 7.3 ± 2.21 mg/dL) and decreased vitamin B12 levels (mean 127.11± 16.16 pg/dL) and folic acid levels (mean 5.96 ± 4.17 ng/dl ) as compared to controls. There was a negative correlation of statistical significance between vitamin B12 levels, folic acid levels and homocysteine levels. Furthermore, there was a statistically significant positive correlation between CRP and homocysteine levels.
Conclusions: There were significantly decreased levels of vitamin B12 and folic acid in children with coeliac disease as compared to controls. There were also significantly increased levels of CRP and serum homocysteine levels along with correlation between vitamin B12 levels, folic acid levels, CRP levels and homocysteine levels.
Sri Lanka Journal of Child Health, 2021; 50(4): 589-594