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Original Articles

Prevalence of SEA and SEB enterotoxin producing methicillin-resistant staphylococcus aureus strains among primary school children in Sari, Iran

Authors:

Sahar Khalili,

Mazandaran University of Medical Sciences, Sari, IR
About Sahar
MSc Student in Microbiology, Student Research Committee, Faculty of Medicine
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Nikou Bahrami,

Shahid Chamran University of Ahvaz, IR
About Nikou
MSc Graduate, Department of Biology, Faculty of Science
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Shaghaygh Rezai,

Mashhad University of Medical Sciences, Mashhad, IR
About Shaghaygh
Department of Microbiology and Virology, Faculty of Medicine
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Iman Pouladi

Lorestan University of Medical Sciences, Khorramabad, IR
About Iman

MSc in Medical Microbiology, Student Research Committee

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Abstract

Background: Staphylococcus aureus is a frequent hospital and community-acquired infection. It has different types of virulence factors and provides host invasion requirements through the release of various toxins, the super-antigenic enterotoxins, SEA and SEB being the most likely to cause pathogenicity.


Objectives:  To assess the prevalence of SEA and SEB enterotoxin producing MRSA strains among primary school children in Sari, northern Iran.


Method: In this descriptive cross-sectional study, 140 nasal isolates of primary school children were collected for 4 months in 2017 in Sari city. First, isolates were identified utilising biochemical and laboratory methods. Minimum inhibitory concentration of isolates to oxacillin was next determined using phenotypic and molecular methods. The presence of SEA and SEB genes were then detected using polymerase chain reaction sequence specific primers (PCR-SSP).


Results: We identified 70 Staphylococcus aureus isolates through standard microbiological procedures. In the phenotypic antibiotic susceptibility assay of 70 isolates of Staphylococcus aureus, 42 isolates were identified as oxacillin- susceptibility assay of 70 isolates of Staphylococcus aureus, 42 isolates were identified as oxacillin-resistant isolates according to the CLSI guidelines, and were reported as methicillin-resistant staphylococcus aureus (MRSA) nasal carriers. Also, 23 cases of oxacillin-resistant isolates lacked the mecA gene (false positive). Nineteen isolates were definite MRSA using PCR, of which 8 isolates (21.42%) had SEA gene and 3 isolates (7.14%) had SEB gene. Also, three isolates (7.14%) carried both the SEA and SEBgenes.


Conclusions: In primary school children in Sari, northern Iran, of the 19 definite MRSA isolates using PCR, 21.4% had the SEA gene, 7.1% had the SEB gene and 7.1% carried both the SEA and SEB genes.


Sri Lanka Journal of Child Health, 2021; 50: 17-21

How to Cite: Khalili, S., Bahrami, N., Rezai, S. and Pouladi, I., 2021. Prevalence of SEA and SEB enterotoxin producing methicillin-resistant staphylococcus aureus strains among primary school children in Sari, Iran. Sri Lanka Journal of Child Health, 50(1), pp.17–21. DOI: http://doi.org/10.4038/sljch.v50i1.9394
Published on 05 Mar 2021.
Peer Reviewed

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